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Optogenetic control of nuclear protein export.
Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B
Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
72655NLS-mCherry-LEXY (pDN122)Blue light-inducible nuclear export of mCherry
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72656pLEXY (pDN137)golden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)
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72657pNLS-LEXY (pDN138)golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)
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72658H2B-GFP-LEXY (pDN157)Constitutively nuclear LEXY variant for blocking endogenous, CRM-1-dependent nuclear export with blue light
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72659H2B-GFP-AsLOV2-NES27 (pDN158)Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export
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72660NLS-mCherry-NES reporter (pDN160)NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158
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72661LEXY TR (without KRAB domain) (pDN162)LexATF-T2A-NLS-LexA-mCherry-LEXY construct for light control of transcriptional repression
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72662LEXY TR (with KRAB domain) (pDN163)LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY construct for light control of transcriptional repression
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