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Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling.
Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H
Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
71879pAC164-pmax-dCas9Master-VP64dCas9-3xGGGGS-VP64 in pmax expression vector
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71881pAC1355-pmax-NLSPUFa_VP64NLSPUFa_VP64 in transient expression vector pmax
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71882pAC1356-pmax-NLSPUFb_VP64NLSPUFb_VP64 in expression vector pmax
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71883pAC1357-pmax-NLSPUFw_VP64NLSPUFw_VP64 in expression vector pmax
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71884pAC1358-pmax-NLSPUFc_VP64NLSPUFc_VP64 in expression vector pmax
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71887pAC1364-pmax-dCas9Master_mCBPHATdCas9Master_mCBPHAT in pmax expression vector
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71888pAC1371-pX-sgRNA-5xPBSaCloning vector for expression of sgRNA-5xPBSa
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71889pAC1372-pX-sgRNA-15xPBSaCloning vector for expression of sgRNA-15xPBSa
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71890pAC1373-pX-sgRNA-25xPBSaCloning vector for expression of sgRNA-25xPBSa
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71891pAC1374-pX-sgRNA-5xPBSbCloning vector for transient expression of sgRNA-5xPBSb
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71892pAC1375-pX-sgRNA-15xPBSbCloning vector for expression of sgRNA-15xPBSb
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71893pAC1376-pX-sgRNA-25xPBSbCloning vector for expression of sgRNA-25xPBSb
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71894pAC1379-pX-sgRNA-5xPBSwCloning vector for expression of sgRNA-5xPBSw
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71895pAC1380-pX-sgRNA-5xPBScCloning vector for expression of sgRNA-5xPBSc
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71897pAC1393-pmax-NLSPUFa_p65HSF1NLSPUFa_p65HSF1 in pmax expression vector
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71898pAC1394-pX-sgRNA-0xPBSCloning vector for expression of sgRNA without PBS. It contains extra sequences for BsaI digestion for insertion of PBS
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71899pAC1399-pX-sgRNA-20xPBScCloning vector for expression of sgRNA-20xPBSc
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71900pAC1402-pCR8-Clover_NLSPUFaClover_NLSPUFa in pCR8 gateway donor vector
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71901pAC1403-pCR8-Clover_NLSPUFcClover_NLSPUFc in pCR8 gateway donor vector
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71902pAC1404-pCR8-mRuby2_NLSPUFamRuby2_NLSPUFa in pCR8 gateway donor vector
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71903pAC1405-pCR8-4xNLS_PUFa_2xNLSNLSPUFa pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest
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71904pAC1406-pCR8-4xNLS_PUFb_2xNLSNLSPUFb pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest
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71905pAC1407-pCR8-4xNLS_PUFw_2xNLSNLSPUFw pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest
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71906pAC1408-pCR8-4xNLS_PUFc_2xNLSNLSPUFc pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest
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71907pAC1410-pmax-dCas9Master_p65HSF1dCas9Master_p65HSF1 in pmax expression vector
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71908pAC1411-pmax-NLSPUFc_p65HSF1NLSPUFc_p65HSF1 in pmax expression vector
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71912pAC1415-pCR8-4xNLS_PUFa_2xNLS_mCBPHAT4xNLS_PUFa_2xNLS_mCBPHAT in pCR8 Gateway donor vector
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71913pAC1416-pCR8-mCBPHAT_4xNLS_PUFa_2xNLSmCBPHAT_4xNLS_PUFa_2xNLS in pCR8 Gateway donor vector
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71914pAC1417-pmax-4xNLS_PUFa_2xNLS_mCBPHAT4xNLS_PUFa_2xNLS_mCBPHAT in pmax expression vector
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71915pAC1418-pmax-mCBPHAT_4xNLS_PUFa_2xNLSmCBPHAT_4xNLS_PUFa_2xNLS in pmax expression vector
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71917pAC1420-pX-sgRNA-1xPBSaCloning vector for expression of sgRNA-1xPBSa
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71918pAC1421-pX-sgRNA-2xPBSaCloning vector for expression of sgRNA-2xPBSa
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71919pAC1422-pX-sgRNA-1xPBSbCloning vector for expression of sgRNA-1xPBSb
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71920pAC1423-pX-sgRNA-2xPBSbCloning vector for expression of sgRNA-2xPBSb
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71921pAC1424-pX-sgRNA-10xPBSbCloning vector for expression of sgRNA-10xPBSb
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71925pAC1425-pX-sgRNA-20xPBSbCloning vector for expression of sgRNA-20xPBSb
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71926pAC1426-pX-sgRNA-47xPBSbCloning vector for expression of sgRNA-47xPBSb
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71927pAC1427-pX-sgRNA-10xPBSwCloning vector for expression of sgRNA-10xPBSw
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71928pAC1428-pX-sgRNA-15xPBSwCloning vector for expression of sgRNA-15xPBSw
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71929pAC1429-pX-sgRNA-10xPBScCloning vector for expression of sgRNA-10xPBSc
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71930pAC1430-pX-sgRNA-15xPBScCloning vector for expression of sgRNA-15xPBSc
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