Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein.
Silas S, Mohr G, Sidote DJ, Markham LM, Sanchez-Amat A, Bhaya D, Lambowitz AM, Fire AZ
Science. 2016 Feb 26;351(6276):aad4234. doi: 10.1126/science.aad4234. PubMed Article
Silas S, Mohr G, Sidote DJ, Markham LM, Sanchez-Amat A, Bhaya D, Lambowitz AM, Fire AZ
Science. 2016 Feb 26;351(6276):aad4234. doi: 10.1126/science.aad4234. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78409 | pV92.2 | pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct |