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Dynamics of Translation of Single mRNA Molecules In Vivo.
Yan X, Hoek TA, Vale RD, Tanenbaum ME
Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74925pHR-PP7-2xmCherry-CAAXExpresses PP7-2xmCherry-CAAX
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74926pHR-tdPP7-3xmCherryExpresses tdPP7-3xmCherry
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74927pcDNA4TO-5xGCN4_v4-kif18b-24xPP7Translation reporter
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74928pcDNA4TO-24xGCN4_v4-kif18b-24xPP7Translation reporter
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74929pcDNA4TO-24xGCN4_v4-BFP-24xPP7Translation reporter
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74931pcDNA4TO-24xGCN4_v4-kif18b-Xbp1-24xPP7Translation reporter with Xbp1 pause site
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74932pcDNA4TO-5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7Translation reporter
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74934pcDNA4TO-24xGCN4_v4-kif18bTranslation reporter that lacks PP7 sites in the 3'UTR
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134833pHRdSV40-UP-24xGCN4-2xIFP2-CAAX-24xPP7Expresses 24xSunTag-2xIFP2-CAAX (24xGCN4_v4-2xIFP2-CAAX)
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