DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for Interactome Mapping in Mammalian Cells.
Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C, Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8. PubMed Article
Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C, Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78855 | pPA-RL-GW | DULIP Bait Destination Vector (Gateway, N-terminal) |
| 78856 | pGW-RL-PA | DULIP Bait Destination Vector (Gateway, C-terminal) |
| 78857 | pFL-V5-GW | DULIP Prey Destination Vector (Gateway, N-terminal) |
| 78858 | pGW-FL-V5 | DULIP Prey Destination Vector (Gateway, C-terminal) |
| 78859 | PA-RL-BAD | DULIP positive control (bait) for assay establishment |
| 78860 | FL-BCL2L1 | DULIP positive control (prey) for assay establishment |
| 78861 | PA-RL-mCherry | DULIP negative control (bait) to exclude unspecific background binding |
| 78862 | FL-mCherry | DULIP negative control (prey) to exclude unspecific background binding |
| 78863 | pPA-RL-FL | DULIP Tandem Construct used for data normalization |