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Long-range transcriptional interference in E. coli used to construct a dual positive selection system for genetic switches.
Hoffmann SA, Kruse SM, Arndt KM
Nucleic Acids Res. 2016 Jun 2;44(10):e95. doi: 10.1093/nar/gkw125. Epub 2016 Feb 29. PubMed Article

Plasmids from Article

ID Plasmid Purpose
79824pSAH0016Dual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicol
79826pBAD_Kan-LacIArabinose induced expression of Lac repressor
79828SAH0141-0.0-conP01Transcriptional interference assessment; strong interfering promoter, landing site for spacers
79830SAH0098-scrPXTranscriptional interference assessment; no interfering promoter (scrambled)
79831SAH0146-0.5-conP01Transcriptional interference assessment; strong interfering promoter, 500 bp spacer

Antibodies from Article