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Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.
Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
72793pMK277 (mClover-NeoR)C-terminal tagging with mClover using CRIPSR/Cas9
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72794pMK278 (mClover-Hygro)C-terminal tagging with mClover using CRIPSR/Cas9
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72795pMK279 (mClover-Bsr)C-terminal tagging with mClover using CRIPSR/Cas9
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72796pMK280 (mCherry2-NeoR)C-terminal tagging with mCherry2 using CRIPSR/Cas9
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72797pMK281 (mCherry2-Hygro)C-terminal tagging with mCherry2 using CRIPSR/Cas9
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72798pMK282 (mCherry2-Bsr)C-terminal tagging with mCherry2 using CRIPSR/Cas9
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72799pMK283 (Stag-3FLAG-Neo)C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9
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72800pMK284 (Stag-3FLAG-Hygro)C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9
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72801pMK285 (Stag-3FLAG-Bsr)C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9
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72824pMK286 (mAID-NeoR)C-terminal tagging with mAID using CRISPR/Cas9
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72825pMK287 (mAID-Hygro)C-terminal tagging with mAID using CRISPR/Cas9
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72826pMK288 (mAID-Bsr)C-terminal tagging with mAID using CRISPR/Cas9
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72827pMK289 (mAID-mClover-NeoR)C-terminal tagging with mAID-mClover using CRISPR/Cas9
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72828pMK290 (mAID-mClover-Hygro)C-terminal tagging with mAID-mClover using CRISPR/Cas9
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72829pMK291 (mAID-mClover-Bsr)C-terminal tagging with mAID-mClover using CRISPR/Cas9
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72830pMK292 (mAID-mCherry2-NeoR)C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9
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72831pMK293 (mAID-mCherry2-Hygro)C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9
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72832pMK294 (mAID-mCherry2-Bsr)C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9
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72833AAVS1 T2 CRIPR in pX330CRISPR/Cas to target the AAVS1 locus in human cells
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72834pMK232 (CMV-OsTIR1-PURO)Expresses OsTIR1 under the control of the CMV promoter
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72835pMK243 (Tet-OsTIR1-PURO)Expresses OsTIR1 under the control of the Tet promoter
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Antibodies from Article