CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. PubMed Article
Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66939 | pCAS9-mCherry-Frame +0 | CRISPaint frame selector +0 |
| 66940 | pCAS9-mCherry-Frame +1 | CRISPaint frame selector +1 |
| 66941 | pCAS9-mCherry-Frame +2 | CRISPaint frame selector +2 |
| 66942 | pCAS9-mCherry-TUBB | CRISPaint target selector TUBB |
| 66943 | pCAS9-mCherry-ACTG1 | CRISPaint target selector ACTG1 |
| 66944 | pCAS9-mCherry-HIST1H4C | CRISPaint target selector HIST1H4C |
| 67166 | pCRISPaint-TagGFP2 | CRISPaint universal donor for TagGFP2 fusion tagging |
| 67167 | pCRISPaint-TagRFP | CRISPaint universal donor for TagRFP fusion tagging |
| 67168 | pCRISPaint-TagBFP | CRISPaint universal donor for TagBFP fusion tagging |
| 67169 | pCRISPaint-TC | CRISPaint universal donor for tetracysteine fusion tagging |
| 67170 | pCRISPaint-3xFlag | CRISPaint universal donor for flag fusion tagging |
| 67171 | pCRISPaint-HA | CRISPaint universal donor for HA fusion tagging |
| 67172 | pCRISPaint-myc | CRISPaint universal donor for myc fusion tagging |
| 67173 | pCRISPaint-SBP | CRISPaint universal donor for streptavidin binding peptide fusion tagging |
| 67174 | pCRISPaint-StrepTag II | CRISPaint universal donor for strep tag II fusion tagging |
| 67175 | pCRISPaint-AviTag | CRISPaint universal donor for AviTag fusion tagging |
| 67176 | pCRISPaint-SpyTag | CRISPaint universal donor for SpyTag fusion tagging |
| 67177 | pCRISPaint-1xSunTag | CRISPaint universal donor for SunTag fusion tagging |
| 67178 | pCRISPaint-NanoLuc | CRISPaint universal donor for NanoLuc fusion tagging |
| 67180 | pCRISPaint-2A-TurboGFP-PEST | CRISPaint universal donor for gene expression quantification using destabilized TurboGFP |
| 67181 | pCRISPaint-ProteoTuner DD | CRISPaint universal donor for fusion tagging allowing inducible destabilization |
| 67182 | pCRISPaint-nTEV | CRISPaint universal donor for split-TEV-N fusion tagging |
| 67183 | pCRISPaint-cTEV | CRISPaint universal donor for split-TEV-C fusion tagging |
| 80956 | pCRISPaint-2A-TurboGFP-PEST-PuroR | CRISPaint gene tagging |
| 80957 | pCRISPaint-AviTag-PuroR | CRISPaint gene tagging |
| 80958 | pCRISPaint-cTEV-PuroR | CRISPaint gene tagging |
| 80959 | pCRISPaint-HA-PuroR | CRISPaint gene tagging |
| 80960 | pCRISPaint-HaloTag-PuroR | CRISPaint gene tagging |
| 80961 | pCRISPaint-myc-PuroR | CRISPaint gene tagging |
| 80962 | pCRISPaint-NanoLuc-PuroR | CRISPaint gene tagging |
| 80963 | pCRISPaint-nTEV-PuroR | CRISPaint gene tagging |
| 80964 | pCRISPaint-ProteoTuner DD-PuroR | CRISPaint gene tagging |
| 80965 | pCRISPaint-SBP-PuroR | CRISPaint gene tagging |
| 80966 | pCRISPaint-SpyTag-PuroR | CRISPaint gene tagging |
| 80967 | pCRISPaint-StrepTag II-PuroR | CRISPaint gene tagging |
| 80968 | pCRISPaint-SunTag-PuroR | CRISPaint gene tagging |
| 80969 | pCRISPaint-TagBFP-PuroR | CRISPaint gene tagging |
| 80970 | pCRISPaint-TagGFP2-PuroR | CRISPaint gene tagging |
| 80971 | pCRISPaint-TagRFP-PuroR | CRISPaint gene tagging |
| 80972 | pCRISPaint-TC-PuroR | CRISPaint gene tagging |
| 80973 | pCRISPaint-3xflag-PuroR | CRISPaint gene tagging |
| 80974 | pRZ-CAS9-mCherry | Cas9, mCherry expression |
| 80975 | pCAS9-mCherry empty | Cas9, mCherry, and sgRNA expression |
| 80976 | pCAS9-BFP empty | Cas9, TagBFP, and sgRNA expression |
| 174090 | pCRISPaint-mNeon-PuroR | CRISPaint gene tagging |
| 174092 | pCRISPaint-mNeon | CRISPaint gene tagging |