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CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
66939pCAS9-mCherry-Frame +0CRISPaint frame selector +0
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66940pCAS9-mCherry-Frame +1CRISPaint frame selector +1
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66941pCAS9-mCherry-Frame +2CRISPaint frame selector +2
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66942 pCAS9-mCherry-TUBBCRISPaint target selector TUBB
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66943pCAS9-mCherry-ACTG1CRISPaint target selector ACTG1
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66944pCAS9-mCherry-HIST1H4CCRISPaint target selector HIST1H4C
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67166pCRISPaint-TagGFP2CRISPaint universal donor for TagGFP2 fusion tagging
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67167pCRISPaint-TagRFPCRISPaint universal donor for TagRFP fusion tagging
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67168pCRISPaint-TagBFPCRISPaint universal donor for TagBFP fusion tagging
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67169pCRISPaint-TCCRISPaint universal donor for tetracysteine fusion tagging
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67170pCRISPaint-3xFlagCRISPaint universal donor for flag fusion tagging
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67171pCRISPaint-HACRISPaint universal donor for HA fusion tagging
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67172pCRISPaint-mycCRISPaint universal donor for myc fusion tagging
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67173pCRISPaint-SBPCRISPaint universal donor for streptavidin binding peptide fusion tagging
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67174pCRISPaint-StrepTag IICRISPaint universal donor for strep tag II fusion tagging
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67175pCRISPaint-AviTagCRISPaint universal donor for AviTag fusion tagging
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67176pCRISPaint-SpyTagCRISPaint universal donor for SpyTag fusion tagging
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67177pCRISPaint-1xSunTagCRISPaint universal donor for SunTag fusion tagging
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67178pCRISPaint-NanoLucCRISPaint universal donor for NanoLuc fusion tagging
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67180pCRISPaint-2A-TurboGFP-PESTCRISPaint universal donor for gene expression quantification using destabilized TurboGFP
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67181pCRISPaint-ProteoTuner DDCRISPaint universal donor for fusion tagging allowing inducible destabilization
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67182pCRISPaint-nTEVCRISPaint universal donor for split-TEV-N fusion tagging
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67183pCRISPaint-cTEVCRISPaint universal donor for split-TEV-C fusion tagging
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80956pCRISPaint-2A-TurboGFP-PEST-PuroRCRISPaint gene tagging
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80957pCRISPaint-AviTag-PuroRCRISPaint gene tagging
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80958pCRISPaint-cTEV-PuroRCRISPaint gene tagging
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80959pCRISPaint-HA-PuroRCRISPaint gene tagging
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80960pCRISPaint-HaloTag-PuroRCRISPaint gene tagging
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80961pCRISPaint-myc-PuroRCRISPaint gene tagging
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80962pCRISPaint-NanoLuc-PuroRCRISPaint gene tagging
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80963pCRISPaint-nTEV-PuroRCRISPaint gene tagging
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80964pCRISPaint-ProteoTuner DD-PuroRCRISPaint gene tagging
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80965pCRISPaint-SBP-PuroRCRISPaint gene tagging
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80966pCRISPaint-SpyTag-PuroRCRISPaint gene tagging
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80967pCRISPaint-StrepTag II-PuroRCRISPaint gene tagging
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80968pCRISPaint-SunTag-PuroRCRISPaint gene tagging
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80969pCRISPaint-TagBFP-PuroRCRISPaint gene tagging
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80970pCRISPaint-TagGFP2-PuroRCRISPaint gene tagging
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80971pCRISPaint-TagRFP-PuroRCRISPaint gene tagging
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80972pCRISPaint-TC-PuroRCRISPaint gene tagging
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80973pCRISPaint-3xflag-PuroRCRISPaint gene tagging
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80974pRZ-CAS9-mCherryCas9, mCherry expression
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80975pCAS9-mCherry emptyCas9, mCherry, and sgRNA expression
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80976pCAS9-BFP emptyCas9, TagBFP, and sgRNA expression
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