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Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, Ram R, Lu TK
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
78934PP255Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.
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78935PP295Expresses GFP. Figure 3. Strain 1.
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78936PP296Expresses GFP. Figure 3. Strain 2.
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78937PP297Expresses GFP. Figure 3. Strain 3.
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78938PP298Expresses GFP. Figure 3. Strain 4.
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78939PP299Expresses GFP. Figure 3. Strain 6.
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78940PP300Expresses GFP. Figure 3. Strain 7.
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78941PP318Expresses GFP. Figure 3. Strain 5.
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78942PP228Expresses GFP. Figures 3 and 4. Strain 8. Promoter: ppTEF1, minimal Promoter: mCYC.
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78943PP322Expresses GFP. Figure 3. Strain 9.
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78944PP74Supplemental Figure 3. Integration Site 1.
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78945PP75Supplemental Figure 3. Integration Site 2.
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78946PP76Supplemental Figure 3. Integration Site 3.
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78947PP77Supplemental Figure 3. Integration Site 4.
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78948PP78Supplemental Figure 3. Integration Site 5.
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78949PP79Supplemental Figure 3. Integration Site 6.
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78950PP69Supplemental Figure 3. Integration Site 7.
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78951PP67Supplemental Figure 3. Integration Site 8.
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78952PP151Supplemental Figure 3. Integration Site 9.
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78953PP43Figure 2. Expresses BxbI.
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78954PP44Figure 2. Expresses R4.
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78955PP45Figure 2. Expresses TP901-1.
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78961PP259Promoter: scTEF1, minimal Promoter: mCYC. Figure 4. Expresses GFP.
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78962PP258Promoter: GAP6, minimal Promoter: mCYC. Figure 4. Expresses GFP.
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78963PP257Promoter: GAP, minimal Promoter: mCYC. Figure 4. Expresses GFP.
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78964PP247Promoter: scTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP.
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78965PP245Promoter: GAP, minimal Promoter: AOX1. Figure 4. Expresses GFP.
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78966PP246Promoter: GAP6, minimal Promoter: AOX1. Figure 4. Expresses GFP.
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78967PP277Promoter: scTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP.
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78968PP275Promoter: GAP, minimal Promoter: GCW14. Figure 4. Expresses GFP.
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78969PP190Promoter: ppTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP.
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78970PP276Promoter: GAP6, minimal Promoter: GCW14. Figure 4. Expresses GFP.
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78971PP273Promoter: ppTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP.
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78972PP263Promoter: scTEF1, minimal Promoter: GAP6. Figure 4. Expresses GFP.
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78973PP261Promoter: GAP, minimal Promoter: GAP6. Figure 4. Expresses GFP.
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78974PP262Promoter: GAP6, minimal Promoter: GAP6. Figure 4. Expresses GFP.
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78975PP254Promoter: GAP6, minimal Promoter: GAP. Figure 4. Expresses GFP.
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78976PP253Promoter: GAP, minimal Promoter: GAP. Figure 4. Expresses GFP.
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78977PP229Promoter: ppTEF1, minimal Promoter: GAP6. Figure 4. Expresses GFP.
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78978PP244Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.
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78979PP310245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.
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78980PP311246R. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.
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78981PP362255R. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses GFP and RFP.
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78982PP324246B. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.
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78983PP326245B. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.
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78984PP363255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.
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78985PP165Supplemental Figure 4. Promoter GCW14. Expresses GFP.
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78986PP152Supplemental Figure 4. Promoter long ppTEF1. Expresses GFP.
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78987PP87Supplemental Figure 4. Promoter scTEF1. Expresses GFP.
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78988PP164Supplemental Figure 4. Promoter short ppTEF1. Expresses GFP.
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78989PP149Supplemental Figure 4. Promoter WT GAP. Expresses GFP.
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78990PP93Supplemental Figure 4. No Promoter.
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78991PP154Supplemental Figure 4. Promoter GAP1. Expresses GFP.
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78992PP160Supplemental Figure 4. Promoter GAP2. Expresses GFP.
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78993PP161Supplemental Figure 4. Promoter GAP3. Expresses GFP.
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78994PP155Supplemental Figure 4. Promoter GAP4. Expresses GFP.
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78995PP162Supplemental Figure 4. Promoter GAP5. Expresses GFP.
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78996PP163Supplemental Figure 4. Promoter GAP6. Expresses GFP.
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78997PP153Supplemental Figure 4. Promoter GAP7. Expresses GFP.
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