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Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer.
Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
83083TET-pLKO.1 PURO shGDF11 #1Lentiviral shRNA vector for inducible knockdown of human GDF11 (cross reacts with mouse Gdf11)
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83084TET-pLKO.1 PURO shGDF11 #2Lentiviral shRNA vector for inducible knockdown of human GDF11
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83085TET-pLKO.1 PURO shGFPLentiviral shRNA vector for inducible knockdown of GFP (control hairpin)
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83086TET-pLKO.1 PURO shID2 #1Lentiviral shRNA vector for inducible knockdown of human ID2
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83087TET-pLKO.1 PURO shID2 #2Lentiviral shRNA vector for inducible knockdown of human ID2
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83088TET-pLKO.1 PURO shId2 #1Lentiviral shRNA vector for inducible knockdown of mouse Id2
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83089TET-pLKO.1 PURO shSMAD4 #1Lentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)
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83090TET-pLKO.1 PURO shId2 #2Lentiviral shRNA vector for inducible knockdown of mouse Id2
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83091TET-pLKO.1 PURO shSMAD4 #2Lentiviral shRNA vector for inducible knockdown of human SMAD4
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83092TET-pLKO.1 PURO shLucLentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)
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83093pEN_TT 3xFLAG LacZGateway entry vector for inducible expression of LacZ with N-terminal 3xFLAG tag
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83094pEN_TT 3xFLAG SMAD4Gateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tag
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83095pBabe neo TGFBR3-HARetroviral vector for constitutive TGFBR3 expression with C-terminal HA tag
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83096pLX302 ID2-V5 puroLentiviral vector for constitutive expression of human ID2 with C-terminal V5 tag
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83097pLX302 GDF11-V5 puroLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag
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83098pLX304 GDF11-V5 blastLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag
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83099pLX304 LacZ-V5 blastLentiviral vector for constitutive expression of LacZ with C-terminal V5 tag
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83100pLX304 PCSK5-V5 blastLentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tag
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83101pLX304 PCSK5 (T288P)-V5 blastLentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag
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83102pLX304 PCSK5 (A270T)-V5 blastLentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag
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83103pLX304 PCSK5 (H516P)-V5 blastLentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag
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83104pLX304 PCSK5 (R511C)-V5 blastLentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag
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83105pSLIK TT 3xFLAG LacZ neoLentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag
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83273pSLIK TT 3xFLAG SMAD4 neoLentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tag
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83274pEN_TT miRc2 3xFLAGEmpty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG
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98389pLX302 PCSK5-V5 puroLentiviral vector for constitutive expression of human PCSK5 with C-terminal V5 tag
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98390pEN_TT 3xFLAG ID2Gateway entry vector for inducible expression of human ID2 with N-terminal 3xFLAG tag
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98391pEN_TT 3xFLAG LuciferaseGateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag
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98392pSLIK TT 3xFLAG Luciferase neoLentiviral vector for inducible expression of Luciferase with 3xFLAG N-terminal tag
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98393pSLIK TT 3xFLAG ID2 neoLentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag
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98394pSLIK TT 3xFLAG ID2 hygroLentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag
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98395pSLIK TT 3xFLAG LacZ hygroLentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag
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98396pBabe NEO VenusRetroviral vector for constitutive Venus expression
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98397pBabe NEO mRFP1Retroviral vector for constitutive mRFP1 expression
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98579pLX302 LacZ-V5 puroLentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag
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98580pLX304 Luciferase-V5 blastLentiviral vector for constitutive expression of Luciferase with C-terminal V5 tag
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98632TET-pLKO.1 PURO shLacZLentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)
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98709pLX302 PCSK5 (T288P)-V5 puroLentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag
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Antibodies from Article