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Substrate Affinity Differentially Influences Protein Kinase C Regulation and Inhibitor Potency.
Sommese RF, Sivaramakrishnan S
J Biol Chem. 2016 Aug 23. pii: jbc.M116.737601. PubMed Article

Plasmids from Article

ID Plasmid Purpose
83563pBiex PKC - Peptide 8 mCit mCerExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
83564pBiex PKC - Peptide 8 GFP RFPExpresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
83565pBiex PKC - Peptide 14 mCit mCerExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.

Antibodies from Article