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Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation.
Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS
Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. PubMed Article

Plasmids from Article

ID Plasmid Purpose
83969hCRISPRi-v2, genome-wide, top 5 sgRNAs/geneVersion 2 of the human CRISPRi genome wide library
83971hCRISPRi-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/geneHuman CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets
83972hCRISPRi-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting cancer and apoptosis genes
83973hCRISPRi-v2, Stress and Proteostasis (h3), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting stress and proteostasis genes
83974hCRISPRi-v2, Mitochondria, Trafficking, and Motility (h4), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting Mitochondria, Trafficking, and Motility genes
83975hCRISPRi-v2, Gene Expression (h5), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting gene expression
83976hCRISPRi-v2, Membrane Proteins (h6), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting membrane proteins
83977hCRISPRi-v2, Unassigned (h7), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting unassigned genes
83978hCRISPRa-v2, genome-wide, top 5 sgRNAs/geneVersion 2 of the human CRISPRa genome wide library
83980hCRISPRa-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/geneHuman CRISPRa Pooled Library tartgeting kinsases, phosphatases and Drug Targets
83981hCRISPRa-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting cancer and apoptosis genes
83982hCRISPRa-v2, Stress and Proteostasis (h3), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting stress and proteostasis genes
83983hCRISPRa-v2, Mitochondria, Trafficking, and Motility (h4), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting Mitochondria, Trafficking, and Motility genes
83984hCRISPRa-v2, Gene Expression (h5), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting gene expression
83985hCRISPRa-v2, Membrane Proteins (h6), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting membrane proteins
83986hCRISPRa-v2, Unassigned (h7), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting unassigned genes
83987mCRISPRi-v2, genome-wide, top 5 sgRNAs/geneVersion 2 of the mouse CRISPRi genome wide library
83989mCRISPRi-v2, Kinases, Phosphatases, and Drug Targets (m1), top 5 sgRNAs/geneMouse CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets
83990mCRISPRi-v2, Cancer and Apoptosis (m2), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting cancer and apoptosis genes
83991mCRISPRi-v2, Stress and Proteostasis (m3), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting stress and proteostasis genes
83992mCRISPRi-v2, Mitochondria, Trafficking, and Motility (m4), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting Mitochondria, Trafficking, and Motility genes
83993mCRISPRi-v2, Gene Expression (m5), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting gene expression
83994mCRISPRi-v2, Membrane Proteins (m6), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting membrane proteins
83995mCRISPRi-v2, Unassigned (m7), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting unassigned genes
83996mCRISPRa-v2, genome-wide, top 5 sgRNAs/geneVersion 2 of the mouse CRISPRa genome wide library
83998mCRISPRa-v2, Kinases, Phosphatases, and Drug Targets (m1), top 5 sgRNAs/geneMouse CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets
83999mCRISPRa-v2, Cancer and Apoptosis (m2), top 5 sgRNAs/geneMouse CRISPRa Pooled Library targeting cancer and apoptosis genes
84000mCRISPRa-v2, Stress and Proteostasis (m3), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting stress and proteostasis genes
84001mCRISPRa-v2, Mitochondria, Trafficking, and Motility (m4), top 5 sgRNAs/geneMouse CRISPRa Pooled Library targeting Mitochondria, Trafficking, and Motility genes
84002mCRISPRa-v2, Gene Expression (m5), top 5 sgRNAs/geneMouse CRISPRa Pooled Library targeting gene expression
84003mCRISPRa-v2, Membrane Proteins (m6), top 5 sgRNAs/geneMouse CRISPRa Pooled Library targeting membrane proteins
84004mCRISPRa-v2, Unassigned (m7), top 5 sgRNAs/geneMouse CRISPRa Pooled Library targeting unassigned genes
84832pCRISPRia-v2CRISPRi/a V2 library parental plasmid

Antibodies from Article