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Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation.
Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS
Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
83969hCRISPRi-v2, genome-wide, top 5 sgRNAs/geneVersion 2 of the human CRISPRi genome wide library
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83971hCRISPRi-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/geneHuman CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets
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83972hCRISPRi-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting cancer and apoptosis genes
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83973hCRISPRi-v2, Stress and Proteostasis (h3), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting stress and proteostasis genes
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83974hCRISPRi-v2, Mitochondria, Trafficking, and Motility (h4), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting Mitochondria, Trafficking, and Motility genes
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83975hCRISPRi-v2, Gene Expression (h5), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting gene expression
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83976hCRISPRi-v2, Membrane Proteins (h6), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting membrane proteins
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83977hCRISPRi-v2, Unassigned (h7), top 5 sgRNAs/geneHuman CRISPRi Pooled Library targeting unassigned genes
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83978hCRISPRa-v2, genome-wide, top 5 sgRNAs/geneVersion 2 of the human CRISPRa genome wide library
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83980hCRISPRa-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/geneHuman CRISPRa Pooled Library tartgeting kinsases, phosphatases and Drug Targets
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83981hCRISPRa-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting cancer and apoptosis genes
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83982hCRISPRa-v2, Stress and Proteostasis (h3), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting stress and proteostasis genes
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83983hCRISPRa-v2, Mitochondria, Trafficking, and Motility (h4), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting Mitochondria, Trafficking, and Motility genes
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83984hCRISPRa-v2, Gene Expression (h5), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting gene expression
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83985hCRISPRa-v2, Membrane Proteins (h6), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting membrane proteins
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83986hCRISPRa-v2, Unassigned (h7), top 5 sgRNAs/geneHuman CRISPRa Pooled Library targeting unassigned genes
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83987mCRISPRi-v2, genome-wide, top 5 sgRNAs/geneVersion 2 of the mouse CRISPRi genome wide library
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83989mCRISPRi-v2, Kinases, Phosphatases, and Drug Targets (m1), top 5 sgRNAs/geneMouse CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets
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83990mCRISPRi-v2, Cancer and Apoptosis (m2), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting cancer and apoptosis genes
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83991mCRISPRi-v2, Stress and Proteostasis (m3), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting stress and proteostasis genes
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83992mCRISPRi-v2, Mitochondria, Trafficking, and Motility (m4), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting Mitochondria, Trafficking, and Motility genes
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83993mCRISPRi-v2, Gene Expression (m5), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting gene expression
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83994mCRISPRi-v2, Membrane Proteins (m6), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting membrane proteins
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83995mCRISPRi-v2, Unassigned (m7), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting unassigned genes
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83996mCRISPRa-v2, genome-wide, top 5 sgRNAs/geneVersion 2 of the mouse CRISPRa genome wide library
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83998mCRISPRa-v2, Kinases, Phosphatases, and Drug Targets (m1), top 5 sgRNAs/geneMouse CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets
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83999mCRISPRa-v2, Cancer and Apoptosis (m2), top 5 sgRNAs/geneMouse CRISPRa Pooled Library targeting cancer and apoptosis genes
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84000mCRISPRa-v2, Stress and Proteostasis (m3), top 5 sgRNAs/geneMouse CRISPRi Pooled Library targeting stress and proteostasis genes
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84001mCRISPRa-v2, Mitochondria, Trafficking, and Motility (m4), top 5 sgRNAs/geneMouse CRISPRa Pooled Library targeting Mitochondria, Trafficking, and Motility genes
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84002mCRISPRa-v2, Gene Expression (m5), top 5 sgRNAs/geneMouse CRISPRa Pooled Library targeting gene expression
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84003mCRISPRa-v2, Membrane Proteins (m6), top 5 sgRNAs/geneMouse CRISPRa Pooled Library targeting membrane proteins
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84004mCRISPRa-v2, Unassigned (m7), top 5 sgRNAs/geneMouse CRISPRa Pooled Library targeting unassigned genes
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84832pCRISPRia-v2CRISPRi/a V2 library parental plasmid
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