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DIGIT Is a Conserved Long Noncoding RNA that Regulates GSC Expression to Control Definitive Endoderm Differentiation of Embryonic Stem Cells.
Daneshvar K, Pondick JV, Kim BM, Zhou C, York SR, Macklin JA, Abualteen A, Tan B, Sigova AA, Marcho C, Tremblay KD, Mager J, Choi MY, Mullen AC
Cell Rep. 2016 Oct 4;17(2):353-365. doi: 10.1016/j.celrep.2016.09.017.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
82695pgRGFPExpresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.
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82696pCRE-iRFP670Expresses CRE and iRFP670 under two separate PGK promoters.
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82697pAAVS1-shRNAFor targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.
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