Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

DIGIT Is a Conserved Long Noncoding RNA that Regulates GSC Expression to Control Definitive Endoderm Differentiation of Embryonic Stem Cells.
Daneshvar K, Pondick JV, Kim BM, Zhou C, York SR, Macklin JA, Abualteen A, Tan B, Sigova AA, Marcho C, Tremblay KD, Mager J, Choi MY, Mullen AC
Cell Rep. 2016 Oct 4;17(2):353-365. doi: 10.1016/j.celrep.2016.09.017.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
82695pgRGFPExpresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.
82696pCRE-iRFP670Expresses CRE and iRFP670 under two separate PGK promoters.
82697pAAVS1-shRNAFor targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.