Dorus Gadella Lab:
Gadella lab plasmids
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98832 | CytERM-mKOkappa | In vivo visualization of the ER (can be used for colocalization studies and the OSER assay) |
| 98833 | CytERM-mTurquoise2 | In vivo visualization of the ER (can be used for colocalization studies and the OSER assay) |
| 98834 | CytERM-dTomato | In vivo visualization of the ER (can be used for colocalization studies and the OSER assay) |
| 112936 | LSS-SGFP2-aTub | Visualization of microtubules |
| 112937 | LSS-SGFP2_H2A | Visualization of histones |
| 112938 | LifeAct-LSS-SGFP2-N1 | Visualization of F-actin |
| 112939 | pRSETb-LSS-SGFP2 | Bacterial expression vector for LSS-SGFP2 |
| 112940 | CytERM-LSS-SGFP2 | In vivo visualization of the ER (can be used for colocalization studies and the OSER assay) |
| 112941 | pRSETb-LssmsfGFP | Bacterial expression vector for LSS-msfGFP |
| 112942 | pRSETb-LSS-mEGFP | Bacterial expression vector for LSS-EGFP |
| 112943 | mCherry-LSS-SGFP2 | Produces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRET |
| 112944 | mCherry-T2A-LSS-SGFP2 | Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET |
| 112945 | mScarlet-I-LSS-SGFP2 | Produces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRET |
| 112946 | mScarlet-I-T2A-LSS-SGFP2 | Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET |
| 112947 | RhoA sensor (LSS-SGFP2-Scarlet-I) | RhoA FRET biosensor |
| 112948 | RhoA sensor (LSS-SGFP2-mCherry) | RhoA FRET biosensor |
| 112949 | pmCherry-Ggamma2 | mCherry-Ggamma2 fusion |
| 112950 | mScarlet-I-Ggamma2 | mScarlet-I-Ggamma2 fusion |
| 112951 | Gaq-LSS-SGFP2 | Galphaq tagged with LSS-SGFP2 |
| 112952 | RhoA sensor (mScarlet-I_10_LSS-SGFP2_10) | RhoA FRET biosensor |
| 112953 | pTfR-mTurquoise2 | In vivo visualization of Transferrin receptors (can be used for colocalization studies) |
| 112954 | pTfR-mScarlet-I | In vivo visualization of Transferrin receptors (can be used for colocalization studies) |
| 112958 | CytERM_3xmTurquoise2 | In vivo visualization of the ER (can be used for colocalization studies and the OSER assay) |
| 130509 | pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet | expresses mScarlet |
| 130510 | pDRESS_mTurquoise2_spatial-linker-P2A_mScarletI | expresses mScarlet1 |
| 137809 | PiggyBac-G13-sensor | A FRET sensor for G13 activation, encoded on a single plasmid |
| 176092 | iRFP670 - H2A | nuclear marker |
| 176093 | miRFP670 - H2A | Nucelear marker |
| 176105 | LifeAct-HaloTag | F-actin marker |
| 176111 | SspB-mCherry-DHPH1-TRIO | Heterodimerization with iLID, Rac1 and RhoG activation |
| 176115 | SspB-HaloTag-DHPH1-TRIO | Heterodimerization with iLID, Rac1 and RhoG activation |
| 176119 | SspB-iRFP-DHPH1-TRIO | Heterodimerization with iLID, Rac1 and RhoG activation |
| 176126 | pLV-SspB-HaloTag-TRIO-DHPH1 | Heterodimerization with iLID, Rac1 and RhoG activation |
| 176133 | pEntr1a-SspB-HaloTag-TRIO-DHPH1 | Heterodimerization with iLID, Rac1 and RhoG activation |