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Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration.
O'Neill PR, Kalyanaraman V, Gautam N
Mol Biol Cell. 2016 May 1;27(9):1442-50. doi: 10.1091/mbc.E15-12-0832. Epub 2016 Mar 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85220ITSN-mcherry-sspBRole of Cdc-42 selective GEF domain from ITSN in immune cell migration
85221mTiam1-mcherry-sspB in pcDNA3.1Role of Rac selective GEF domain from Tiam1 in immune cell migration
85222Venus wGBDVenus tagged Cdc-42 sensor
85223PH Akt-VenusVenus tagged PH domain of Akt for use as a PIP3 sensor
85680ILID-CAAXExpression of untagged ILID CAAX