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An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting.
Cao J, Wu L, Zhang SM, Lu M, Cheung WK, Cai W, Gale M, Xu Q, Yan Q
Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85400Lenti-iCas9-neoLentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
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85401Lenti-multi-GuideLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
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85402Lenti-multi-CRISPRLentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression
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85745Lenti-entry-puroLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.
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