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Rob Mitra Lab: Mitra lab plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
86949pAAV-short Cre-on G/WGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAV
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86950AAV-short_BrokenHeart (no BC)AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.
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86954pAAV_GWRev_DestGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)
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86955pAAV_GW_DestGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.
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138616ADH1pro_CBF1_SIR4 Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
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138617ADH1pro_TYE7_SIR4 Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
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138618ADH1pro_GCR1_SIR4 Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
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138619ADH1pro_GCR2_SIR4 Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
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138623TEF2pro_mCherry_YFPDual reporter backbone plasmid for CCRA experiment
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138624TEF1pro_mCherry_YFPDual reporter backbone plasmid for CCRA experiment
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