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Designed proteins induce the formation of nanocage-containing extracellular vesicles.
Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King NP, Sundquist WI
Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30. PubMed Article

Plasmids from Article

ID Plasmid Purpose
79916pCMV-EPN-01Myr1-12GS-I3-01-myc-Late1
79917pCMV-EPN-02Myr1_G2A-12GS-I3-01-myc-Late1
79918pCMV-EPN-03Myr2-12GS-I3-01-myc-Late1
79919pCMV-EPN-04Myr3-12GS-I3-01-myc-Late1
79920pCMV-EPN-05Myr4-12GS-I3-01-myc-Late1
79921pCMV-EPN-06Myr1-Late1-I3-01-myc
79922pCMV-EPN-07Palm1-12GS-I3-01-myc-Late1
79923pCMV-EPN-08Palm2-12GS-I3-01-myc-Late1
79924pCMV-EPN-09Palm3-12GS-I3-01-myc-Late1
79925pCMV-EPN-10Palm4-12GS-I3-01-myc-Late1
79926pCMV-EPN-11Palm1-Late1-I3-01-myc
79927pCMV-EPN-12I3-01p-myc-Late1-CaaX1
79928pCMV-EPN-13Late1-I3-01-myc-22GS-CaaX1
79929pCMV-EPN-14Late1-I3-01-myc-22GS-CaaX2
79930pCMV-EPN-15Late1-I3-01-myc-22GS-CaaX3
79931pCMV-EPN-16Late1-I3-01-myc-22GS-CaaX4
79932pCMV-EPN-17PH-4GS-I3-01-myc
79933pCMV-EPN-18PH-4GS-I3-01-myc-Late1
79934pCMV-EPN-19Late1-I3-01-myc-PH
79935pCMV-EPN-20Late2-4GS-I3-01-myc-PH
79936pCMV-EPN-21Late11-I3-01-myc-PH
79937pCMV-EPN-22Late12-I3-01-myc-PH
79938pCMV-EPN-23Late1-PH-4GS-I3-01-myc
79939pCMV-EPN-24PH-Late1-I3-01-myc
79940pCMV-EPN-25I3-01p-myc-Late1-PH
79941pCMV-EPN-26I3-01p-myc-10GS-PH-Late1
79942pCMV-EPN-27C1-10GS-I3-01-myc
79943pCMV-EPN-28C1-10GS-I3-01-myc-Late1
79944pCMV-EPN-29Late1-I3-01-myc-10GS-C1
79945pCMV-EPN-30C2-22GS-I3-01-myc
79946pCMV-EPN-31C2-22GS-I3-01-myc-Late1
79947pCMV-EPN-32Late1-I3-01-myc-22GS-C2
79948pCMV-EPN-33PX-10GS-I3-01-myc
79949pCMV-EPN-34PX-10GS-I3-01-myc-Late1
79950pCMV-EPN-35Late1-I3-01-myc-4GS-PX
79951pCMV-EPN-36Myr1-12GS-I3-01-myc-Late2
79952pCMV-EPN-37Myr1-12GS-I3-01-myc-Late3
79953pCMV-EPN-38Myr1-12GS-I3-01-myc-Late4
79954pCMV-EPN-39Myr1-12GS-I3-01-myc-22GS-Late1 (modified)
79955pCMV-EPN-40Myr1-12GS-I3-01-myc-22GS-Late2 (modified)
79956pCMV-EPN-41Myr1-12GS-I3-01-myc-22GS-Late3
79957pCMV-EPN-42Myr1-12GS-I3-01-myc-22GS-Late4 (modified)
79958pCMV-EPN-43Myr1-12GS-I3-01-myc-22GS-Late5 (modified)
79959pCMV-EPN-44Myr1-12GS-I3-01-myc-22GS-Late6 (modified)
79960pCMV-EPN-45Myr1-12GS-I3-01-myc-22GS-Late7 (modified)
79961pCMV-EPN-46Myr1-12GS-I3-01-myc-22GS-Late8 (modified)
79962pCMV-EPN-47Myr1-12GS-I3-01-myc-22GS-Late9 (modified)
79963pCMV-EPN-48Myr1-12GS-I3-01-myc-22GS-Late10 (modified)
79964pCMV-EPN-49Myr1_G2A-4GS-O3-33-myc-Late1
79965pCMV-EPN-50Myr1-4GS-O3-33-myc-Late1
79966pCMV-EPN-51Late1-O3-33-myc-PH
80038pCMV-I3-01-MycExpression of I3-01
80039pCMV-EPN-01*Expression of EPN-01*
80040pCMV-EPN-01*(ΔM)Expression or G2A mutant of EPN-01*
80041pCMV-EPN-01*(ΔI)Expression of EPN-01 assembly mutant L33R
80042pCMV-EPN-01*(ΔL1)Expression of PTAP L domain mutant EPN-01*
80043pCMV-EPN-01*(ΔL2)Expression of YPxL L domain mutantd EPN-01*
80044pCMV-EPN-01*(ΔL1+L2)Expression of EPN-01* mutated in both L domains
80045pCMV-EPN-01*-(ΔLF)Expression of Vpr binding mutant EPN-01*
80046pCMV-EPN-01expression of EPN-01
80047pCMV-EPN-01(ΔM)Expression or G2A mutants of EPN-01
80048pCMV-EPN-01(ΔI)Expression of EPN-01 assembly mutant L33R
80049pCMV-EPN-01(ΔL1)Expression of EPN-01 mutated in TSG101 binding site.
80050pCMV-EPN-01(ΔL2)Expression of EPN-01 mutated in ALIX binding site.
80051pCMV-EPN-01(ΔL1+L2)Expression of EPN-01 mutated in both L domains
80052pCMV-EPN-01-(ΔLF)Expression of Vpr binding mutant EPN-01
80053pMM310-MycExpression of a Myc tagged version of the BlaM-Vpr fusion protein
80054pCMV-VSV-G-MycExpression of a Myc tagged VSV-G protein
80055pCMV-VSV-G(P127D)-MycExpression of a Myc tagged VSV-G protein
80104pCMV-EPN-03 (G2A)Myr2(G2A)-12GS-I3-01-myc-Late1
80105pCMV-EPN-07 (2AA3)Palm1(2AA3)-12GS-I3-01-myc-Late1
80106pCMV-EPN-08 (2AA3)Palm2(2AA3)-12GS-I3-01-myc-Late1
80107pCMV-EPN18 (R40A)PH(R40A)-4GS-I3-01-myc-Late1
80108pCMV-EPN51 (L1L2)Late1(delta)-O3-33-myc-PH
80109pCMV-EPN51 (R40A)Late1-O3-33-myc-PH(R40A)
80110pCMV-EPN51 (A167R)Late1-O3-33(A167R)-myc-PH
80111pCMV-EPN11 (L1L2)Palm1-Late1(delta)-I3-01-myc
80112pCMV-EPN20 (deltaL)Late2(delta)-4GS-I3-01-myc-PH
80113pCMV-EPN23 (L1L2)Late1(delta)-PH-4GS-I3-01-myc
80114pCMV-EPN24 (L1L2)PH-Late1(delta)-I3-01-myc
80115pCMV-EPN25 (L1L2)I3-01p-myc-Late1(delta)-PH
80116pCMV-EPN-36 (deltaL)Myr1-12GS-I3-01-myc-Late2(delta)
80117pCMV-EPN-37 (deltaL)Myr1-12GS-I3-01-myc-Late3(delta)
80118pCMV-EPN-41 (deltaL)Myr1-12GS-I3-01-myc-22GS-Late3(delta)
80119pCMV-EPN-01 (1wa3)Myr1-12GS-I3-01-myc-Late1 (1wa3)
80120pCMV-EPN-03 (1wa3)Myr2-12GS-I3-01-myc-Late1 (1wa3)
80121pCMV-EPN-07 (1wa3)Palm1-12GS-I3-01-myc-Late1 (1wa3)
80122pCMV-EPN-11 (1wa3)Palm1-Late1-I3-01-myc (1wa3)
80123pCMV-EPN-18 (1wa3)PH-4GS-I3-01-myc-Late1 (1wa3)
80124pCMV-EPN-25 (1wa3)I3-01p-myc-Late1-PH (1wa3)
80351pEGFP-VSP4-E228QExpression of GFP-tagged, dominant negative VPS4 mutant
80423pET29b-EPN-01*For expression of EPN-01* in E. coli
83374pCMV-Myc-GFP-Vpr-RRExpression of Myc tagged GFP-Vpr fusion protein
83375pCMV-Myc-GFPExpression of Myc tagged GFP
84509pET29b-EPN01*-HisE.coli version of EPN01*
84510pET29b-EPN01(∆M)-HisE.coli version of EPN01(∆M)
84511pET29b-EPN01(∆L1+∆L2)-HisE.coli version of EPN01(∆L1+∆L2)

Antibodies from Article