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Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans.
Arras SD, Chua SM, Wizrah MS, Faint JA, Yap AS, Fraser JA
PLoS One. 2016 Oct 6;11(10):e0164322. doi: 10.1371/journal.pone.0164322. eCollection 2016. PubMed Article

Plasmids from Article

ID Plasmid Purpose
128354pSDMA65Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.
128355pSDMA66gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.
128356pSDMA67gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

Antibodies from Article