Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library.
Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu XS, Wei W
Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31. PubMed Article
Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu XS, Wei W
Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89638 | pLenti-sgRNA-lib 2.0 | sgRNA expression in mammalian cells after Gateway cloning |
| 89639 | scaffold-linker-U6 | construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter. |
| 89640 | Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs) | Paired sgRNA library containing 12,472 pgRNAs targeting 671 human long non-coding RNAs (lncRNAs) |