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Regulation of mRNA translation during mitosis.
Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD
Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957. PubMed Article

Plasmids from Article

ID Plasmid Purpose
67923pHR-mCherry-P2A-strepII-PPPCBExpresses mCherry and strepII-tag-PP2aBeta in mammalian cells
67924pHR_dSV40-Aurora A-GFPExpresses Aurora A-GFP in mammalian cells
67925pHR-mCherry-Emi1_control UTRsExpresses mCherry-Emi1 with control UTRs in mammalian cells
67927pHR-mCherry-Emi1_ARHGAP5 UTRsExpresses mCherry-Emi1 with ARHGAP5 UTRs in mammalian cells
67928pHR_dSV40-H2B-GFPExpresses H2B-GFP in mammalian cells
67929pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ control UTRsTranslational reporter - control UTRs
67930pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Emi1 5' and 3'UTRTranslational reporter - Emi1 UTRs
67931pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTRTranslational reporter - ARHGAP5 UTRs
67932pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTRTranslational reporter - Lox 5'UTR

Antibodies from Article