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Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Staahl BT, Benekareddy M, Coulon-Bainier C, Banfal AA, Floor SN, Sabo JK, Urnes C, Munares GA, Ghosh A, Doudna JA
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
88914pMJ915v2+Nterm Spe1 +Cterm Age1 siteFor expression of modified SpyCas9 in e.coli.
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889151xNLS-pMJ915v2For expression of modified SpyCas9 in e.coli.
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889162xNLS-pMJ915v2For expression of modified SpyCas9 in e.coli.
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889174xNLS-pMJ915v2For expression of modified SpyCas9 in e.coli.
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88918pMJ915v2 + sfGFPFor expression of modified SpyCas9 in e.coli.
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889191xNLS-pMJ915v2-sfGFPFor expression of modified SpyCas9 in e.coli.
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889202xNLS-pMJ915v2-sfGFPFor expression of modified SpyCas9 in e.coli.
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889214xNLS-pMJ915v2-sfGFPFor expression of modified SpyCas9 in e.coli.
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889227xNLS-pMJ915v2-sfGFPFor expression of modified SpyCas9 in e.coli.
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