Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci.
Canver MC, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern EN, Needleman AJ, Galacteros F, Brugnara C, Kutlar A, McKenzie C, Reid M, Chen DD, Das PP, A Cole M, Zeng J, Kurita R, Nakamura Y, Yuan GC, Lettre G, Bauer DE, Orkin SH
Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793. PubMed Article
Canver MC, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern EN, Needleman AJ, Galacteros F, Brugnara C, Kutlar A, McKenzie C, Reid M, Chen DD, Das PP, A Cole M, Zeng J, Kurita R, Nakamura Y, Yuan GC, Lettre G, Bauer DE, Orkin SH
Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87155 | lenti-Cas9-VQR-Blast | lentiviral expression of SpCas9-VQR (NGA PAM restricted) |
| 87156 | lenti-Cas9-VQR-GFP_activity_reporter | This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted). |