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Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci.
Canver MC, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern EN, Needleman AJ, Galacteros F, Brugnara C, Kutlar A, McKenzie C, Reid M, Chen DD, Das PP, A Cole M, Zeng J, Kurita R, Nakamura Y, Yuan GC, Lettre G, Bauer DE, Orkin SH
Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793.
PubMed Article

Plasmids from Article

ID Plasmid  
87155lenti-Cas9-VQR-Blast
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87156lenti-Cas9-VQR-GFP_activity_reporter
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