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Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing.
Goldfarb KC, Cech TR
Genes Dev. 2017 Jan 1;31(1):59-71. doi: 10.1101/gad.286963.116. Epub 2017 Jan 23.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87189px330-MRPsg1disruption of human RMRP gene via CRISPR-Cas9, guide 1
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87190px330-MRPsg2disruption of human RMRP gene via CRISPR-Cas9, guide 2
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87191px330-MRPsg3disruption of human RMRP gene via CRISPR-Cas9, guide 3
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87192px330-MRPsg4disruption of human RMRP gene via CRISPR-Cas9, guide 4
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87193psglessClonePXused for downstream cloning, contains CMV promoter with NheI and XhoI cloning sites flanking a partial insert that gets excised when adding an insert of choice
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87194p_3xFLAG-Rpp25Expression of ectopic 3xFLAG-Rpp25 construct from CMV promoter
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87199p-715MRPprom-MRPcucucExpression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency
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