DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells.
Harterink M, da Silva ME, Will L, Turan J, Ibrahim A, Lang AE, van Battum EY, Pasterkamp RJ, Kapitein LC, Kudryashov D, Barres BA, Hoogenraad CC, Zuchero JB
Nat Methods. 2017 Apr 10. doi: 10.1038/nmeth.4257. PubMed Article
Harterink M, da Silva ME, Will L, Turan J, Ibrahim A, Lang AE, van Battum EY, Pasterkamp RJ, Kapitein LC, Kudryashov D, Barres BA, Hoogenraad CC, Zuchero JB
Nat Methods. 2017 Apr 10. doi: 10.1038/nmeth.4257. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89445 | pCMV-DeAct-GS1 | Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells |
| 89446 | pCMV-DeAct-SpvB | Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells |
| 89453 | pTetON-EGFP | Tet/Dox-inducible expression of EGFP in mammalian cells (control) |
| 89454 | pTetON-DeAct-GS1 | Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells |
| 89455 | pTetON-DeAct-SpvB | Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells |
| 89463 | pTetON-DHFRdd-SpvB; CMV-mCherry | Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter) |