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CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis.
Chen Y, Wang Z, Ni H, Xu Y, Chen Q, Jiang L
Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.
PubMed

Plasmids from Article

ID Plasmid Purpose  
91707pHEE901CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR
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91708pHSE901CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR
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91709pBSE901CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance
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91713pXSE901BCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance
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91714pXSE901BGCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance
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Antibodies from Article