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SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette.

Dickinson D, Slabodnick M, Chen A, Goldstein B
MicroPubl Biol. 2018 May 1;2018:10.17912/W2KT0N. doi: 10.17912/W2KT0N. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
91821pDD399AID CT-tag linker donor for the SapTrap cloning system
91822pDD397AID FP-slot donor for the SapTrap cloning system
91823pDD377Codon-optimized mTurquoise2 donor for the SapTrap cloning system
91824pDD372Codon-optimized GFP donor for the SapTrap cloning system
91825pDD375Codon-optimized mKate2 donor for the SapTrap cloning system
91826pMS050Codon-optimized mScarlet-I donor for the SapTrap cloning system
91827pDD378Codon-optimized HaloTag donor for the SapTrap cloning system
91828pDD401Pmyo-2::GFP FP-slot donor for the SapTrap cloning system
91829pDD363LoxP-flanked SEC donor for the SapTrap cloning system
91830pDD382Lox2272-flanked SEC donor for the SapTrap cloning system
91831pDD364Lox511I-flanked SEC donor for the SapTrap cloning system
91832pDD398AID NT-tag linker donor for the SapTrap cloning system
91833pDD121Cas9 expression in C. elegans
91834pDD379SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

Antibodies from Article