A transcription activator-like effector (TALE) induction system mediated by proteolysis.
Copeland MF, Politz MC, Johnson CB, Markley AL, Pfleger BF
Nat Chem Biol. 2016 Apr;12(4):254-60. doi: 10.1038/nchembio.2021. Epub 2016 Feb 8. PubMed Article
Copeland MF, Politz MC, Johnson CB, Markley AL, Pfleger BF
Nat Chem Biol. 2016 Apr;12(4):254-60. doi: 10.1038/nchembio.2021. Epub 2016 Feb 8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92016 | p5A0-T | Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease |
| 92017 | p5A1-T | Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease |
| 92018 | p5A1-Ti | Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease |
| 92019 | p5A3-T | Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease |
| 92020 | p5A7-T | Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease |
| 92021 | p5B3-T | Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease |
| 92022 | p5C3-T | Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease |
| 92023 | p5mCherry | mCherry reporter plasmid with lac operator in its promoter |
| 92024 | p5sfGFP | sfGFP reporter plasmid with lac operator in its promoter |
| 92025 | p5lysAsfGFP | sfGFP reporter plasmid with lysA TALE binding site in its promoter |
| 92026 | p5sucAmCherry | mCherry reporter plasmid with sucA TALE binding site in its promoter |