Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities.
Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG
Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8. PubMed Article
Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG
Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92379 | Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A | CRISPRa |
| 92380 | Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set B | CRISPRa |
| 92383 | Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF), Set A | CRISPRa |
| 92384 | Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF), Set B | CRISPRa |
| 92385 | Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A | Crispri |
| 92386 | Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set B | Crispri |
| 96922 | pXPR_501 | for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64 |
| 96923 | pXPR_502 | for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF |
| 96925 | pXPR_050 | lentiviral expression of gRNA scaffold |
| 104090 | Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050, Set A | Crispri |
| 104091 | Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050, Set B | Crispri |