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Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9.
Qin P, Parlak M, Kuscu C, Bandaria J, Mir M, Szlachta K, Singh R, Darzacq X, Yildiz A, Adli M
Nat Commun. 2017 Mar 14;8:14725. doi: 10.1038/ncomms14725.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
101152sg2.02 MUC4.1gRNA with two MCP binding sites
101153sg14x(MS2) MUC4.1gRNA with 14 MCP binding site
101154sg2.02 16x(MS2) MUC4.1gRNA with 16 MCP binding site
101155sg2.0 (PP7) Locus#2gRNA with two PCP binding sites
101160MCP-YFPto express MCP-YFP fusion protein
101161PCP-GFPto epress PCP-GFP fusion protein