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Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9.
Qin P, Parlak M, Kuscu C, Bandaria J, Mir M, Szlachta K, Singh R, Darzacq X, Yildiz A, Adli M
Nat Commun. 2017 Mar 14;8:14725. doi: 10.1038/ncomms14725.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
101152sg2.02 MUC4.1gRNA with two MCP binding sites
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101153sg14x(MS2) MUC4.1gRNA with 14 MCP binding site
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101154sg2.02 16x(MS2) MUC4.1gRNA with 16 MCP binding site
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101155sg2.0 (PP7) Locus#2gRNA with two PCP binding sites
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101160MCP-YFPto express MCP-YFP fusion protein
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101161PCP-GFPto epress PCP-GFP fusion protein
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