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Profiling Subcellular Protein Phosphatase Responses to Coxsackievirus B3 Infection of Cardiomyocytes.
Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 10.1074/mcp.O116.063487. Epub 2017 Feb 7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87770pLX302-MKP5-V5 puroExpresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)
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87771pLX302-MKP7-V5 puroExpresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16)
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87772pLX302-DUSP19-V5 puroExpresses V5 tagged human DUSP19
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87773pLX302-JSP1-V5 puroExpresses V5 tagged human JSP1 (DUSP22)
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87774pLX302-PTP-SL-V5 puroExpresses V5 tagged human PTP-SL
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87775pLX302-NIPP1-V5 puroExpresses V5 tagged human NIPP1
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87776pEN_TTmiRc2 3XFLAG-MKK4-EE-NLSUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro
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87777pEN_TTmiRc2 3XFLAG-MKK7-EE-NLSUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro
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87778pEN_TTmiRc2 3XFLAG-EGFP-NLSUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro
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87779pSLIK 3XFLAG-MKK4-EE-NLS hygroInducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence
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87780pSLIK 3XFLAG-MKK7-EE-NLS hygroInducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence
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87781pSLIK 3XFLAG-EGFP-NLS hygroInducible expression of EGFP with a C-terminal NLS sequence
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87790tet-pLKO shMKP1.v1 puroExpresses an inducible short hairpin targeting human MKP1 sequence
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87791tet-pLKO shMKP1.v2 puroExpresses an inducible short hairpin targeting human MKP1 sequence
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87792tet-pLKO shMKP5.v1 puroExpresses an inducible short hairpin targeting human MKP5 sequence
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87793tet-pLKO shMKP5.v2 puroExpresses an inducible short hairpin targeting human MKP5 sequence
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87794tet-pLKO shMKP7.v1 puroExpresses an inducible short hairpin targeting human MKP7 sequence
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87795tet-pLKO shMKP7.v2 puroExpresses an inducible short hairpin targeting human MKP7 sequence
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87796tet-pLKO shDUSP19 puroExpresses an inducible short hairpin targeting human DUSP19 sequence
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