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Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria.
Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, Navarro-Rodriguez M, Rubio LM, Caro E
BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 10.1186/s12896-017-0393-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
101682pUPD-pGAL1 (01-11)pGAL1 in domestication vector
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101683pUPD-pTDH3 (01-11)pTDH3 in domestication vector
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101684pUPD-pTDH2 (01-11)pTDH2 in domestication vector
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101685pUPD-pTEF2 (01-11)pTEF2 in domestication vector
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101686pUPD-pHXT7 (01-11)pHXT7 in domestication vector
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101687pUPD-pTPI1 (01-11)pTPI1 in domestication vector
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101688pUPD-pPYK1 (01-11)pPYK1 in domestication vector
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101689pUPD-PGK1 (01-11)pPGK1 in domestication vector
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101690pUPD-PGI1 (01-11)pPGI1 in domestication vector
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101691pUPD-Su9MTS (12)Su9MTS in domestication vector
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101692pUPD-SOD2MTS (12)SOD2MTS in domestication vector
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101693pUPD-MAM33MTS (12)MAM33MTS in domestication vector
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101694pUPD2-ODPAMTS (12)ODPAMTS in domestication vector
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101695pUPD2-ODPBMTS (12)ODPBMTS in domestication vector
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101696pUPD2-ATPAMTS (12)ATPAMTS in domestication vector
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101697pUPD2-GLRX2MTS (12)GLRX2MTS in domestication vector
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101698pUPD-MTS2 (12)MTS2 in domestication vector
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101699pUC57-8xHis8xHis in domestication vector
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101700pUPD-Cyc1t (17-21)Cyc1t in domestication vector
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101701pUPD-ADH2t (17-21)ADH2t in domestication vector
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101702pUPD-TDH2t (17-21)TDH2t in domestication vector
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101703pUPD-G418R (01-21)G418R in domestication vector
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101704pUPD-HygroBR (01-21)HygroBR in domestication vector
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101705YORWΔ22Ω2GoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci
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101706YORWΔ22Ω1GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci
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101707YORWΔ22α2GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci
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101708YORWΔ22α1GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci
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101709YPRCΔ15Ω2GoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci
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101710YPRCΔ15Ω1GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci
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101711YPRCΔ15α2GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci
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101712YPRCΔ15α1GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci
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