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Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes.
Vigouroux A, Oldewurtel E, Cui L, Bikard D, van Teeffelen S
Mol Syst Biol. 2018 Mar 8;14(3):e7899.
PubMed

Plasmids from Article

ID Plasmid Purpose  
101803pCRRNA-G0-R0Repress mCherry and GFP independently, with different strengths
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101804pCRRNA-G10-R0Repress mCherry and GFP independently, with different strengths
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101805pCRRNA-G11-R0Repress mCherry and GFP independently, with different strengths
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101806pCRRNA-G14-R0Repress mCherry and GFP independently, with different strengths
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101807pCRRNA-G20-R0Repress mCherry and GFP independently, with different strengths
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101808pCRRNA-G0-R11Repress mCherry and GFP independently, with different strengths
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101809pCRRNA-G10-R11Repress mCherry and GFP independently, with different strengths
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101810pCRRNA-G11-R11Repress mCherry and GFP independently, with different strengths
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101811pCRRNA-G14-R11Repress mCherry and GFP independently, with different strengths
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101812pCRRNA-G20-R11Repress mCherry and GFP independently, with different strengths
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101813pCRRNA-G0-R18Repress mCherry and GFP independently, with different strengths
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101814pCRRNA-G10-R18Repress mCherry and GFP independently, with different strengths
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101815pCRRNA-G11-R18Repress mCherry and GFP independently, with different strengths
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101816pCRRNA-G14-R18Repress mCherry and GFP independently, with different strengths
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101817pCRRNA-G20-R18Repress mCherry and GFP independently, with different strengths
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101818pCRRNA-G0-R20Repress mCherry and GFP independently, with different strengths
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101819pCRRNA-G10-R20Repress mCherry and GFP independently, with different strengths
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101820pCRRNA-G11-R20Repress mCherry and GFP independently, with different strengths
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101821pCRRNA-G14-R20Repress mCherry and GFP independently, with different strengths
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101822pCRRNA-G20-R20Repress mCherry and GFP independently, with different strengths
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