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Continuous directed evolution of aminoacyl-tRNA synthetases.
Bryson DI, Fan C, Guo LT, Miller C, Soll D, Liu DR
Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
99147pDB007(+)Accessory plasmid for PACE of MjTyrRS variants
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99209pDB021CH(+)Accessory plasmid for PACE of PylRS variants
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99210pDB038Accessory plasmid for PACE of PylRS variants
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99211pDB038aAccessory plasmid for PACE of PylRS variants
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99212pDB038bAccessory plasmid for PACE of PylRS variants
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99214pDB007ns2aAccessory plasmid for PACE of PylRS variants; negative selection
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99215pDB036aAccessory plasmid for PACE of MjTyrRS variants; negative selection
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99216pDB036dAccessory plasmid for PACE of MjTyrRS variants; negative selection
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99217pDB023fComplementary plasmid for aaRS PACE; amber codons in T7 RNAP
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99218pDB023f1Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
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99219pDB023kComplementary plasmid for aaRS PACE; amber codons in T7 RNAP
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99220pDB016Accessory plasmid for aaRS PACE; negative selection
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99222pTECH-chPylRS(IPYE)Expression plasmid encoding chPylRS(IPYE) and pylT
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99397pDB070.iodo.5Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine
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104069pTECH-chAcK3RS(IPYE)Expression plasmid encoding chAcK3RS(IPYE) and pylT
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104070pTECH-MbAcK3RS(IPYE)Expression plasmid encoding MbAcK3RS(IPYE) and pylT
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104071pTECH-MmAcK3RS(IPYE)Expression plasmid encoding MmAcK3RS(IPYE) and pylT
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104072pTECH-MbPylRS(IPYE)Expression plasmid encoding MbPylRS(IPYE) and pylT
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104073pTECH-MmPylRS(IPYE)Expression plasmid encoding MmPylRS(IPYE) and pylT
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