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Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.
Gu B, Posfai E, Rossant J
Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
103882PCS2+Cas9-mSACas9 mSA for in vitro transcription for gene editing in Mouse Embryos
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113831EasyFusion mAID-mCherry with NeoEasyFusion vector for targeting endogeneous genes with mAID-mCherry
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113832PBS Actb-2A-TIR1-GFPtargeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation
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122948PCS2+ Cas9For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos
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