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Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.
Gu B, Posfai E, Rossant J
Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166.
PubMed Article

Plasmids from Article

ID Plasmid  
103882PCS2+Cas9-mSA
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113831EasyFusion mAID-mCherry with Neo
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113832PBS Actb-2A-TIR1-GFP
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122948PCS2+ Cas9
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