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RNA editing with CRISPR-Cas13.
Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
103849pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.
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103851pC0040-LwaCas13a crRNA backboneBackbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA
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103852pC0041-RanCas13b crRNA backboneFor cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.
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103853pC0042-PguCas13b crRNA backboneFor cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
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103854pC0043-PspCas13b crRNA backboneFor cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
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103855pC0044-EF1a-RanCas13b-NES-mapkExpresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm
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103861pC0045-EF1a-PguCas13b-NES-HIVExpresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm
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103862pC0046-EF1a-PspCas13b-NES-HIVExpresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm
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103863pC0047-CMV-dPspCas13b-ADAR1DD(E1008Q)dPspCas13b-ADAR1DD(E1008Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA
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103864pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
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103865pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058ACatalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
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103866pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
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103867pC0051-W85X REPAIR targeting guide cloned into pC0043Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038
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103868pC0052-REPAIR non-targeting guide clone into pC0043Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.
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103869pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
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103870pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.
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103871pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
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