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Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization.
Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski SM, Gunawardane RN
Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87420AICSDP-1:PXN-EGFPHomology arms and linker-EGFP sequence for C-terminus tagging of human PXN
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87421AICSDP-4:TUBA1B-mEGFPHomology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B
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87422AICSDP-10:LMNB1-mEGFPHomology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
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87423AICSDP-8:TOMM20-mEGFPHomology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20
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87424AICSDP-9:DSP-mEGFPHomology arms and linker-mEGFP sequence for C-terminus tagging of human DSP
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87425AICSDP-15:ACTB-mEGFPHomology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB
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87426AICSDP-7:SEC61B-mEGFPHomology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B
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87427AICSDP-13:FBL-mEGFPHomology arms and linker-mEGFP sequence for C-terminus tagging of human FBL
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87428AICSDP-24:MYH10-mEGFPHomology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10
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87429AICSDP-23:TJP1-mEGFPHomology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1
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