Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on protein stability that destabilise the actin-tropomyosin copolymer in vitro and in vivo.
Johnson CA, Brooker HR, Gyamfi I, O'Brien J, Ashley B, Brazier JE, Dean A, Embling J, Grimsey E, Tomlinson AC, Wilson EG, Geeves MA, Mulvihill DP
Biochem Biophys Res Commun. 2017 Oct 25. pii: S0006-291X(17)32087-9. doi: 10.1016/j.bbrc.2017.10.109. PubMed Article
Johnson CA, Brooker HR, Gyamfi I, O'Brien J, Ashley B, Brazier JE, Dean A, Embling J, Grimsey E, Tomlinson AC, Wilson EG, Geeves MA, Mulvihill DP
Biochem Biophys Res Commun. 2017 Oct 25. pii: S0006-291X(17)32087-9. doi: 10.1016/j.bbrc.2017.10.109. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99360 | pJC20cdc8.A18T | Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr. |
| 99361 | pJC20cdc8.E31K | Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys. |
| 99362 | pJC20cdc8.27 | Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27. |
| 99363 | pJC20cdc8.110 | Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110. |