Intersubunit Crosstalk in the Rag GTPase Heterodimer Enables mTORC1 to Respond Rapidly to Amino Acid Availability.
Shen K, Choe A, Sabatini DM
Mol Cell. 2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 10.1016/j.molcel.2017.09.026. PubMed Article
Shen K, Choe A, Sabatini DM
Mol Cell. 2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 10.1016/j.molcel.2017.09.026. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99641 | pETDuet RagA-RagC | Bacterial expression of wildtype Rag GTPase dimer |
| 99642 | pETDuet RagA(D130N)-RagC | Bacterial expression of RagA(D130N)-RagC |
| 99643 | pETDuet RagA-RagC(D181N) | Bacterial expression of RagA-RagC(D181N) |
| 99644 | pETDuet RagA(T21N)-RagC | Bacterial expression of RagA(T21N)-RagC |
| 99645 | pETDuet RagA-RagC(S75N) | Bacterial expression of RagA-RagC(S75N) |
| 99646 | pETDuet RagA(R37P)-RagC | Bacterial expression of RagA(R37P)-RagC |
| 99647 | pETDuet RagA-RagC(L91P) | Bacterial expression of RagA-RagC(L91P) |
| 99648 | pETDuet RagA-RagC(K84T)) | Bacterial expression of RagA-RagC(K84T) |
| 99649 | pK RagA-RagC | Bacterial expression of wildtype Rag GTPase dimer |
| 99650 | pK RagA(T21N)-RagC | Bacterial expression of RagA(T21N)-RagC |
| 99699 | pK RagA-RagC(S75N) | Bacterial expression of RagA-RagC(S75N) |
| 99700 | pK RagA(Q66L)-RagC | Bacterial expression of RagA(Q66L)-RagC |
| 99701 | pK RagA-RagC(Q120L) | Bacterial expression of RagA-RagC(Q120L) |
| 99702 | pK RagA(Q66L)-RagC(Q120L) | Bacterial expression of RagA(Q66L)-RagC(Q120L) |
| 99706 | pK RagA(R37P)-RagC | Bacterial expression of RagA(R37P)-RagC |
| 99707 | pK RagA-RagC(L91P) | Bacterial expression of RagA-RagC(L91P) |
| 99708 | pK RagA-RagC(K84T) | Bacterial expression of RagA-RagC(K84T) |
| 99709 | pRK5 HA-Nprl2 | Mammalian expression of HA-Nprl2 |
| 99710 | pRK5 HA-RagA | Mammalian expression of HA-RagA |
| 99711 | pRK5 HA-RagA(T21N) | Mammalian expression of HA-RagA(T21N) |
| 99712 | pRK5 HA-RagA(Q66L) | Mammalian expression of HA-RagA(Q66L) |
| 99713 | pRK5 HA-RagA(R37P) | Mammalian expression of HA-RagA(R37P) |
| 99714 | pRK5 Flag-RagA | Mammalian expression of Flag-RagA |
| 99715 | pRK5 Flag-RagA(T21N) | Mammalian expression of Flag-RagA(T21N) |
| 99716 | pRK5 Flag-RagA(Q66L) | Mammalian expression of Flag-RagA(Q66L) |
| 99717 | pRK5 Flag-RagA(R37P) | Mammalian expression of Flag-RagA(R37P) |
| 99718 | pRK5 HA-RagC | Mammalian expression of HA-RagC |
| 99719 | pRK5 HA-RagC(S75N) | Mammalian expression of HA-RagC(S75N) |
| 99720 | pRK5 HA-RagC(Q120L) | Mammalian expression of HA-RagC(Q120L) |
| 99721 | pRK5 HA-RagC(L91P) | Mammalian expression of HA-RagC(L91P) |
| 99722 | pRK5 HA-RagC(K84T) | Mammalian expression of HA-RagC(K84T) |
| 99723 | pRK5 Flag-RagC | Mammalian expression of Flag-RagC |
| 99724 | pRK5 Flag-RagC(S75N) | Mammalian expression of Flag-RagC(S75N) |
| 99725 | pRK5 Flag-RagC(Q120L) | Mammalian expression of Flag-RagC(Q120L) |
| 99726 | pRK5 Flag-RagC(L91P) | Mammalian expression of Flag-RagC(L91P) |
| 99727 | pRK5 Flag-RagC(K84T) | Mammalian expression of Flag-RagC(K84T) |