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Expression of IgG Monoclonals with Engineered Immune Effector Functions.
Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D
Methods Mol Biol. 2018;1827:313-334. doi: 10.1007/978-1-4939-8648-4_16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
104579pRVL-1Contains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.
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104580pRVL-2Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions
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104581pRVL-3Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
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104582pRVL-4Contains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.
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104583pRVL-5Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions
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104584pRVL-6Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
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Recombinant Antibodies from Article