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A high-conductance chemo-optogenetic system based on the vertebrate channel Trpa1b.
Lam PY, Mendu SK, Mills RW, Zheng B, Padilla H, Milan DJ, Desai BN, Peterson RT
Sci Rep. 2017 Sep 19;7(1):11839. doi: 10.1038/s41598-017-11791-z. PubMed

Plasmids from Article

ID Plasmid Purpose
106291pME-Trpa1b-2A-mCherryGateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherry
106403pCMV-Trpa1b-FLAGMammalian expression vector containing zebrafish Trpa1b with C-terminal FLAG tag.
106405Tol2-cmlc2-Trpa1b-2A-EGFPFor expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.
106425Tol2-ngn1-Trpa1b-2A-mCherryFor expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

Antibodies from Article