Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Robust transcriptional activation in plants using multiplexed CRISPR-Act2.0 and mTALE-Act systems.
Lowder LG, Zhou J, Zhang Y, Malzahn A, Zhong Z, Hsieh TF, Voytas DF, Zhang Y, Qi Y
Mol Plant. 2017 Nov 29. pii: S1674-2052(17)30344-1. doi: 10.1016/j.molp.2017.11.010.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
99325pYPQ121Express two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
Loading...
99882pFUS_A8Recipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.
Loading...
99883pYPQ127BExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoter
Loading...
99884pYPQ131A2.0Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
Loading...
99885pYPQ131B2.0Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
Loading...
99886pYPQ131C2.0Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter
Loading...
99887pYPQ132A2.0Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
Loading...
99888pYPQ132B2.0Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
Loading...
99889pYPQ132C2.0Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter
Loading...
99890pYPQ132D2.0Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
Loading...
99891pYPQ133A2.0Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
Loading...
99892pYPQ133B2.0Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
Loading...
99893pYPQ133C2.0Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter
Loading...
99894pYPQ133D2.0Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
Loading...
99895pYPQ140Empty vector with attL5-attL2 recombination sites for Gateway Cloning
Loading...
99896pYPQ141A2.0Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
Loading...
99897pYPQ141B2.0Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
Loading...
99905pYPQ141C2.0Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter
Loading...
99906pYPQ141D2.0Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
Loading...
99907pYPQ173Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
Loading...
100044pYPQ131D2.0Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
Loading...