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Robust transcriptional activation in plants using multiplexed CRISPR-Act2.0 and mTALE-Act systems.
Lowder LG, Zhou J, Zhang Y, Malzahn A, Zhong Z, Hsieh TF, Voytas DF, Zhang Y, Qi Y
Mol Plant. 2017 Nov 29. pii: S1674-2052(17)30344-1. doi: 10.1016/j.molp.2017.11.010.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
99325pYPQ121Express two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
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99882pFUS_A8Recipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.
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99883pYPQ127BExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoter
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99884pYPQ131A2.0Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
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99885pYPQ131B2.0Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
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99886pYPQ131C2.0Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter
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99887pYPQ132A2.0Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
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99888pYPQ132B2.0Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
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99889pYPQ132C2.0Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter
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99890pYPQ132D2.0Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
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99891pYPQ133A2.0Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
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99892pYPQ133B2.0Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
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99893pYPQ133C2.0Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter
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99894pYPQ133D2.0Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
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99895pYPQ140Empty vector with attL5-attL2 recombination sites for Gateway Cloning
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99896pYPQ141A2.0Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
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99897pYPQ141B2.0Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
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99905pYPQ141C2.0Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter
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99906pYPQ141D2.0Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
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99907pYPQ173Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
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100044pYPQ131D2.0Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
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Antibodies from Article