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Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons.
Fernandopulle MS, Prestil R, Grunseich C, Wang C, Gan L, Ward ME
Curr Protoc Cell Biol. 2018 Jun;79(1):e51. doi: 10.1002/cpcb.51. Epub 2018 May 18. PubMed Article

Plasmids from Article

ID Plasmid Purpose
105840pUCM-AAVS1-TO-hNGN2Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron
105841pUCM-CLYBL-hNILDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
105842CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

Antibodies from Article