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Fluorescence-based quantitative synapse analysis for cell-type specific connectomics.
Kuljis DA, Park E, Telmer CA, Lee J, Ackerman DS, Bruchez MP, Barth AL
eNeuro. 2019 Sep 23. pii: ENEURO.0193-19.2019. doi: 10.1523/ENEURO.0193-19.2019.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
105981pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGHThis AAV plasmid constitutively expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
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105982pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
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105983pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
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Antibodies from Article