Time-gated detection of protein-protein interactions with transcriptional readout.
Kim MW, Wang W, Sanchez MI, Coukos R, von Zastrow M, Ting AY
Elife. 2017 Nov 30;6. doi: 10.7554/eLife.30233.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104839 | pAAV-UAS-CITRINE | SPARK reporter gene |
| 104840 | pAAV-UAS-Luciferase | SPARK reporter gene |
| 104841 | pAAV-b2AR-eLOV-TEVcs-FLAG-GAL4-V5 | b2AR-SPARK TF component |
| 104842 | pAAV-b2AR-hLOV1-TEVcs-FLAG-GAL4-V5 | b2AR-SPARK TF component |
| 104843 | pAAV-NMBR-eLOV-TEVcs-FLAG-GAL4-V5 | b2AR-SPARK TF component |
| 104844 | pAAV-AVPR2-eLOV-TEVcs-FLAG-GAL4-V5 | b2AR-SPARK TF component |
| 104845 | pAAV-beta-arrestin2-HA-TEV219 | b2AR-SPARK protease component |
| 104846 | pLX208-beta-arrestin2-HA-TEV219 | b2AR-SPARK protease component |