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Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish.
Zhou W, Cao L, Jeffries J, Zhu X, Staiger CJ, Deng Q
Dis Model Mech. 2018 Mar 28;11(3). pii: dmm.033027. doi: 10.1242/dmm.033027.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107590Tol2-lyzC-mito-Dendra2Expresses mitochondria localized Dendra2 in zebrafish neutrophils
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107591p5E-lyzCAn entry vector with a lyzC promoter
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107592pME-cas9-2A-mCherryAn entry vector with cas9 -2A-mCherry
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107593p3E-U6aAn entry vector with U6a promoter driving guide RNA expression
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107594pCS2-sod1Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
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107598p3E-U6a-polg guideAn entry vector with U6a promoter driving polg guide RNA expression
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107599p3E-U6a-U6cAn entry vector with U6a and U6c promoter driving guide RNAs expression
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107600p3E-U6a-U6c-rac2 guideAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expression
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107601p3E-U6a-U6c-ndufs2 guideAn entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression
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107602p3E-U6a-U6c-uqcrc1 guideAn entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression
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107603p3E-U6a-U6c-sod1 guideAn entry vector with U6a and U6c promoter driving sod1 guide RNAs expression
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107604p3E-U6a-U6c-sod2 guideAn entry vector with U6a and U6c promoter driving sod2 guide RNAs expression
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