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Synergistic assembly of human pre-spliceosomes across introns and exons.
Braun JE, Friedman LJ, Gelles J, Moore MJ
Elife. 2018 Jun 22;7. pii: 37751. doi: 10.7554/eLife.37751. PubMed Article

Plasmids from Article

ID Plasmid Purpose
106986pcDNA5-FRT-TetO-fSNAPcopen reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites
106987pcDNA5-FRT-TetO-U1-70K-fSNAPcopen reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites
106988pcDNA5-FRT-TetO-U2B''-fSNAPcopen reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites
106989pcDNA5-FRT-TetO-Snu114-fSNAPcopen reading frame of Snu114 inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites
106990T7-3e5T7 transcription template for 3e5
106991T7-5iXT7 transcription template for 5iX
106992T7-Xi3T7 transcription template for Xi3
106993T7-3eXT7 transcription template for 3eX
106994T7-Xe5T7 transcription template for Xe5
106995T7-5i3e5T7 transcription template for 5i3e5
106996T7-5i3eXT7 transcription template for 5i3eX
106997T7-Xi3e5T7 transcription template for Xi3e5
106998T7-Xi3eXT7 transcription template for Xi3eX

Antibodies from Article