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Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production.
Gassaway BM, Cardone RL, Padyana AK, Petersen MC, Judd ET, Hayes S, Tong S, Barber KW, Apostolidi M, Abulizi A, Sheetz JB, Kshitiz, Aerni HR, Gross S, Kung C, Samuel VT, Shulman GI, Kibbey RG, Rinehart J
Cell Rep. 2019 Dec 10;29(11):3394-3404.e9. doi: 10.1016/j.celrep.2019.11.009.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107155MBP-rPKL S12TAGMBP-rPKL construct for production and purification of rat PKL pS12
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107156MBP-rPKL WTMBP-rPKL WT for production and purification of rat PKL WT
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107157MBP-rPKL S113TAGMBP-rPKL S113TAG for production and purification of rat PKL pS113
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107158MBP-rPKM2 WTMBP-rPKM2 WT for production and purification of rat PKM2 WT
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107159Flag-hPKL WTFor mammalian expression of Flag-hPKL WT
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107160Flag-hPKL S113AFor mammalian expression of Flag-hPKL S113A
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107161Flag-hPKL S113DFor mammalian expression of Flag-hPKL S113D
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Antibodies from Article