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LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in Acute Myeloid Leukemia.
Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, Klingbeil O, El Demerdash O, Shi J, Vakoc CR
Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
108098LRG2.1Lentiviral expression plasmid of sgRNA with GFP
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108099LRCherry2.1Lentiviral expression plasmid of sgRNA with mCherry
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108100LentiV_Cas9_puroLentiviral expression plasmid of spCas9 with puromycin resistance gene
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108101LentiV_NeoLentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)
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108103LentiV_Neo_SIK3Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene
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108104LentiV_Neo_SIK3_CRLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene
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108106LentiV_Neo_SIK3_CR_K95MLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance gene
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108107LentiV_Neo_SIK3_CR_T221ALentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance gene
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108108LentiV_Neo_SIK3_CR_T221ELentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene
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108109LentiV_Neo_SIK3_CR_T142QLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance gene
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108110LentiV_Neo_SIK3_T142QLentiviral expression plasmid of human SIK3 cDNA (gatekeeper mutation) with neomycin resistance gene
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108111LentiV_Neo_LKB1Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene
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108112LentiV_Neo_LKB1_CRLentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene
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117725Human Kinase Domain-Focused CRISPR Knockout LibrarygRNAs targeting the kinase domain of human protein kinases
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Antibodies from Article