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Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors.
Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA
Nat Chem Biol. 2018 Nov 26. pii: 10.1038/s41589-018-0168-3. doi: 10.1038/s41589-018-0168-3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
108251sAJM.1504Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B
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108253sAJM.1505Marionette-Pro. Marionette cluster inserted in the direction of leading strand replication, between 3,720,027 and 3,721,644 in E. coli BL21
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108254sAJM.1506Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655
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108512pAJM.711PPhlF-YFP Reporter. DAPG sensor for use in Marionette strain.
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108513pAJM.712PCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.
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108514pAJM.713PLuxB-YFP Reporter. OC6 sensor for use in Marionette strain.
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108515pAJM.714PVanCC-YFP Reporter. Van sensor for use in Marionette strain.
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108516pAJM.715PTac-YFP Reporter. IPTG sensor for use in Marionette strain.
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108517pAJM.717PTet*-YFP Reporter. aTc sensor for use in Marionette strain.
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108518pAJM.716PBAD-YFP Reporter. Ara sensor for use in Marionette strain.
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108519pAJM.718PBetI-YFP Reporter. Cho sensor for use in Marionette strain.
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108520pAJM.719PTtg-YFP Reporter. Nar sensor for use in Marionette strain.
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108521pAJM.1459P3B5C-YFP Reporter. DHBA sensor for use in Marionette strain.
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108522pAJM.721PSalTTC-YFP Reporter. Sal sensor for use in Marionette strain.
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108523pAJM.944PCin-YFP Reporter. OHC14 sensor for use in Marionette strain.
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108524pAJM.847PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.
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108525pAJM.657CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.
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108526pAJM.474LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.
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108527pAJM.773VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.
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108528pAJM.336LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.
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108529pAJM.011TetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain.
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108530pAJM.677AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.
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108531pAJM.683BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.
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108532pAJM.661TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.
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108533pAJM.690PcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.
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108534pAJM.771NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.
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108535pAJM.1642CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.
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108536pAJM.884AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.
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108537pAJM.969MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.
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